| 100 Times Too Many (secret) | Get killed by the Alien 100 times | Bronze |
| A Hunt Begins | Complete the third mission | Bronze |
| A Perfect Organism | Encounter the Alien in Sevastopol for the first time | Bronze |
| A Record of Disaster | Collect an archive log | Bronze |
| A Synthetic Solution | Complete the twelfth mission | Bronze |
| A True Engineer | Construct one of each craftable item | Bronze |
| Alien Isolation | Unlocked every Alien | Isolation trophy | Platinum |
| An Outpost of Progress | Complete the seventh mission | Bronze |
| Archivist | Collect 10 Nostromo logs in the main campaign | Bronze |
| Awake | Complete the first mission | Bronze |
| Back Off (secret) | Cause the Alien to retreat by using the flamethrower | Bronze |
| Bait | Complete the tenth mission | Bronze |
| Build to Survive | Construct an item | Bronze |
| Caught in the Trap | Complete the sixth mission | Bronze |
| Consultation | Complete the thirteenth mission | Bronze |
| End of the Hunt | Complete the eighteenth mission | Bronze |
| Every Bullet Counts | Use the revolver | Bronze |
| Fault Detected | Kill an Android | Bronze |
| Free the Torrens | Complete the seventeenth mission | Bronze |
| Hazard Containment | Complete the eleventh mission | Bronze |
| Hide. Run. Survive. | Complete the fifth mission without being killed by the Alien | Silver |
| How Do You Feel? | Complete the fifth mission | Bronze |
| I Admire its Purity | Detect 30 targets with the motion tracker | Bronze |
| Just out of Reach | Contact your team and escape Comms without being attacked by an android | Bronze |
| Light 'em Up | Use the flamethrower | Bronze |
| Mercy or Prudence? | Complete the game without killing any humans | Gold |
| Mind Your Step (secret) | Navigate Reactor Maintenance without dying | Bronze |
| My Turn Now | Kill an android using only the maintenance jack | Silver |
| Not a Scratch | Escape from android combat without taking damage | Bronze |
| Not the First (secret) | Turn off the beacon | Bronze |
| One Shot | Complete the game without dying | Gold |
| Power Games | Access 10 different rewire points | Bronze |
| Retreat From Fire (secret) | Cause the Alien to retreat using a molotov | Bronze |
| Ripley, Signing Off | Complete the game on any difficulty setting | Silver |
| Seegson Security Bypass | Perform 10 successful hacks | Bronze |
| Seegson Systems Expert | Complete 10 minigames successfully | Bronze |
| Self Defense | Kill 10 humans | Bronze |
| She's in the Vents... | Use the vent system 20 times | Bronze |
| Shock to the System | Use the stun baton | Bronze |
| Stunned (secret) | Knock down a human or stun an android with a non-lethal attack | Bronze |
| Survivor | Complete the game on the hardest difficulty setting | Gold |
| The Message | Complete the fifteenth mission | Bronze |
| The Missing | Collect an ID tag | Bronze |
| The Taken | Collect all ID tags | Silver |
| This Should Work | Use the bolt gun | Bronze |
| Throwing the Switch | Complete the fourteenth mission | Bronze |
| Transmission | Complete the sixteenth mission | Bronze |
| Use With Caution... | Use the shotgun | Bronze |
| Voices of Sevastopol | Collect 100 archive logs | Silver |
| Welcome to Sevastopol | Complete the second mission | Bronze |
| You Shouldn't Be There. | Complete the fourth mission | Bronze |
